Cloned DNA fragment specifying major outer membrane protein a in Escherichia coli K-12
نویسندگان
چکیده
منابع مشابه
Genetic locus (ompB) affecting a major outer-membrane protein in Escherichia coli K-12.
Three multiply colicin-tolerant mutants in Escherichia coli K-12 from the TolIV, TolXIV, and TolXV phenotypic groups, all lacking or having only trace amounts of protein 1, a major outer-membrane protein, were mapped by Hfr crosses, and the position on the chromosome was confirmed by cotransduction with nearby markers. The mutations were located near malQP in the 74-min region of the E. coli ch...
متن کاملPrimary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12.
The amino acid sequence of major outer membrane protein II (ompA protein) from Escherichia coli K-12 has been determined. The transmembrane polypeptide consists of 325 residues, resulting in a molecular weight of 35,159. The transmembrane part of the protein is located between residues 1 and 177. In this part of the protein a predominantly lipophilic 27-residue segment exists that perhaps spans...
متن کاملRole of a major outer membrane protein in Escherichia coli.
Mutants of Escherichia coli B/r lacking a major outer membrane protein, protein b, were obtained by selecting for resistance to copper. These mutants showed a decreased ability to utilize a variety of metabolites when the metabolites were present at low concentrations. Also, mutants of E. coli K-12 lacking proteins b and c from the outer membrane were shown to have an identical defect in the up...
متن کاملEscherichia coli outer membrane protein K is a porin.
Protein K is an outer membrane protein found in pathogenic encapsulated strains of Escherichia coli. We present evidence here that protein K is structurally and functionally related to the E. coli K-12 porin proteins (OmpF, OmpC, and PhoE). Protein K was found to cross-react with antibody to OmpF protein and to share 8 out of 17 peptides in common with the OmpF protein. Strains that are OmpC po...
متن کاملRole of micF in the tolC-mediated regulation of OmpF, a major outer membrane protein of Escherichia coli K-12.
Mutation in the tolC locus greatly reduces normal synthesis of OmpF, a major porin protein of Escherichia coli K-12. Experiments that use ompF-ompC chimeric genes demonstrate that a tolC mutation exerts its effect at either the promoter or the amino-terminal end of the ompF gene. Direct analysis of ompF mRNA from tolC+ and tolC strains showed that the amount of ompF transcript in the latter was...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1978
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.136.1.369-380.1978